Transcription Profile Analysis of Chlorophyll Biosynthesis in Leaves of Wild-Type and Chlorophyll b-Deficient Rice (Oryza sativa L.)

Photosynthesis is an essential biological process and a key approach for raising crop yield. However, photosynthesis in rice not fully investigated. This study reported the photosynthetic properties transcriptomic profiles of chlorophyll (Chl) b-deficient mutant (ch11) wild-type (Oryza sativa L.). Chl revealed irregular chloroplast development (indistinct membranes, loss starch granules, thinner grana, numerous plastoglobuli). Next-generation sequencing application that differential expressed genes were related to machinery, Chl-biosynthesis, degradation pathway ch11. Two encoding PsbR (PSII core protein), FtsZ1, PetH genes, found be down-regulated. The expression FtsZ1 resulted disrupted cell division electron flow, respectively, consequently reducing accumulation capacity rice. Furthermore, this up-regulated GluRS gene, whereas POR gene was down-regulated biosynthesis pathways. results obtained from RT-qPCR analyses generally consistent with those transcription analysis, exception finding MgCH which enhance important intermediate products Mg branch biosynthesis. These indicate reduction both b. suggested decline caused by formation down-regulation genes; b might degraded via pheophorbide pathway, requires further elucidation.